Rapid detection of avian influenza virus H5N1 in chicken tracheal samples using an impedance aptasensor with gold nanoparticles for signal amplification.
Identifieur interne : 000414 ( Main/Exploration ); précédent : 000413; suivant : 000415Rapid detection of avian influenza virus H5N1 in chicken tracheal samples using an impedance aptasensor with gold nanoparticles for signal amplification.
Auteurs : Sardar Karash [États-Unis] ; Ronghui Wang [États-Unis] ; Lisa Kelso [États-Unis] ; Huaguang Lu [États-Unis] ; Tony Jun Huang [États-Unis] ; Yanbin Li [États-Unis]Source :
- Journal of virological methods [ 1879-0984 ] ; 2016.
Descripteurs français
- KwdFr :
- Animaux, Aptamères nucléotidiques (métabolisme), Grippe chez les oiseaux (diagnostic), Grippe chez les oiseaux (virologie), Impédance électrique, Microélectrodes, Nanoparticules (métabolisme), Poulets, Sensibilité et spécificité, Sous-type H5N1 du virus de la grippe A (isolement et purification), Techniques de biocapteur (), Trachée (virologie).
- MESH :
- diagnostic : Grippe chez les oiseaux.
- isolement et purification : Sous-type H5N1 du virus de la grippe A.
- métabolisme : Aptamères nucléotidiques, Nanoparticules.
- virologie : Grippe chez les oiseaux, Trachée.
- Animaux, Impédance électrique, Microélectrodes, Poulets, Sensibilité et spécificité, Techniques de biocapteur.
English descriptors
- KwdEn :
- Animals, Aptamers, Nucleotide (metabolism), Biosensing Techniques (methods), Chickens, Electric Impedance, Influenza A Virus, H5N1 Subtype (isolation & purification), Influenza in Birds (diagnosis), Influenza in Birds (virology), Microelectrodes, Nanoparticles (metabolism), Sensitivity and Specificity, Trachea (virology).
- MESH :
- chemical , metabolism : Aptamers, Nucleotide.
- diagnosis : Influenza in Birds.
- isolation & purification : Influenza A Virus, H5N1 Subtype.
- metabolism : Nanoparticles.
- methods : Biosensing Techniques.
- virology : Influenza in Birds, Trachea.
- Animals, Chickens, Electric Impedance, Microelectrodes, Sensitivity and Specificity.
Abstract
Highly pathogenic avian influenza virus H5N1 is a continuous threat to public health and poultry industry. The recurrence of the H5N1 led us to develop a robust, specific, and rapid detection method for the virus. In this study, an impedance aptasensor was developed for the virus detection using specific H5N1 aptamer and a gold interdigitated microelectrode. Streptavidin was immobilized on the microelectrode surface and biotin labeled H5N1 aptamer was bound to the immobilized streptavidin. The microelectrode was blocked with the polyethylene glycol and the bound aptamer captured the virus. The impedance change caused by the captured virus was measured using an impedance analyzer. To enhance impedance signal, a nanoparticle-based amplifier was designed and implemented by forming a network-like gold nanoparticles/H5N1-aptamer/thiocyanuric acid. The detection limit of the impedance aptasensor was 0.25 HAU for the pure virus and 1 HAU for the tracheal chicken swab samples spiked with the H5N1 virus. The detection time of aptasensor without employing the amplifier was less than an hour. The amplifier increased impedance by a 57-fold for the 1 HAU samples. Only negligible impedance change was observed for non-target viruses such as H5N2, H5N3, H7N2, H1N1, and H2N2. This aptasensor provides a foundation for the development of a portable aptasensor instrument.
DOI: 10.1016/j.jviromet.2016.07.018
PubMed: 27452670
Affiliations:
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Electronic address: yanbinli@uark.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Cell and Molecular Biology Program, University of Arkansas, Fayetteville, AR 72701, USA; Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA; Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701</wicri:regionArea><wicri:noRegion>AR 72701</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of virological methods</title><idno type="eISSN">1879-0984</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Aptamers, Nucleotide (metabolism)</term><term>Biosensing Techniques (methods)</term><term>Chickens</term><term>Electric Impedance</term><term>Influenza A Virus, H5N1 Subtype (isolation & purification)</term><term>Influenza in Birds (diagnosis)</term><term>Influenza in Birds (virology)</term><term>Microelectrodes</term><term>Nanoparticles (metabolism)</term><term>Sensitivity and Specificity</term><term>Trachea (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Aptamères nucléotidiques (métabolisme)</term><term>Grippe chez les oiseaux (diagnostic)</term><term>Grippe chez les oiseaux (virologie)</term><term>Impédance électrique</term><term>Microélectrodes</term><term>Nanoparticules (métabolisme)</term><term>Poulets</term><term>Sensibilité et spécificité</term><term>Sous-type H5N1 du virus de la grippe A (isolement et purification)</term><term>Techniques de biocapteur ()</term><term>Trachée (virologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Aptamers, Nucleotide</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Influenza in Birds</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Grippe chez les oiseaux</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A Virus, H5N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Sous-type H5N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Nanoparticles</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Aptamères nucléotidiques</term><term>Nanoparticules</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe chez les oiseaux</term><term>Trachée</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Influenza in Birds</term><term>Trachea</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chickens</term><term>Electric Impedance</term><term>Microelectrodes</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Impédance électrique</term><term>Microélectrodes</term><term>Poulets</term><term>Sensibilité et spécificité</term><term>Techniques de biocapteur</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Highly pathogenic avian influenza virus H5N1 is a continuous threat to public health and poultry industry. The recurrence of the H5N1 led us to develop a robust, specific, and rapid detection method for the virus. In this study, an impedance aptasensor was developed for the virus detection using specific H5N1 aptamer and a gold interdigitated microelectrode. Streptavidin was immobilized on the microelectrode surface and biotin labeled H5N1 aptamer was bound to the immobilized streptavidin. The microelectrode was blocked with the polyethylene glycol and the bound aptamer captured the virus. The impedance change caused by the captured virus was measured using an impedance analyzer. To enhance impedance signal, a nanoparticle-based amplifier was designed and implemented by forming a network-like gold nanoparticles/H5N1-aptamer/thiocyanuric acid. The detection limit of the impedance aptasensor was 0.25 HAU for the pure virus and 1 HAU for the tracheal chicken swab samples spiked with the H5N1 virus. The detection time of aptasensor without employing the amplifier was less than an hour. The amplifier increased impedance by a 57-fold for the 1 HAU samples. Only negligible impedance change was observed for non-target viruses such as H5N2, H5N3, H7N2, H1N1, and H2N2. This aptasensor provides a foundation for the development of a portable aptasensor instrument.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Pennsylvanie</li></region><settlement><li>University Park (Pennsylvanie)</li></settlement><orgName><li>Université d'État de Pennsylvanie</li></orgName></list><tree><country name="États-Unis"><noRegion><name sortKey="Karash, Sardar" sort="Karash, Sardar" uniqKey="Karash S" first="Sardar" last="Karash">Sardar Karash</name></noRegion><name sortKey="Huang, Tony Jun" sort="Huang, Tony Jun" uniqKey="Huang T" first="Tony Jun" last="Huang">Tony Jun Huang</name><name sortKey="Kelso, Lisa" sort="Kelso, Lisa" uniqKey="Kelso L" first="Lisa" last="Kelso">Lisa Kelso</name><name sortKey="Li, Yanbin" sort="Li, Yanbin" uniqKey="Li Y" first="Yanbin" last="Li">Yanbin Li</name><name sortKey="Lu, Huaguang" sort="Lu, Huaguang" uniqKey="Lu H" first="Huaguang" last="Lu">Huaguang Lu</name><name sortKey="Wang, Ronghui" sort="Wang, Ronghui" uniqKey="Wang R" first="Ronghui" last="Wang">Ronghui Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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